To save crystallization data
نویسندگان
چکیده
منابع مشابه
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Fis protein and DNA Fis, under the control of the T7 RNA polymerase (Pan et al. 1996), was expressed by auto-induction (Studier 2005). Extracts were prepared using a French Press and nucleic acids were removed by precipitation with 0.35% polyethyleneimine followed by precipitation with ammonium sulfate (80% saturation). Fis was purified by FPLC chromatography over a Bioscale S20 (Bio-Rad) colum...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section D Biological Crystallography
سال: 2001
ISSN: 0907-4449
DOI: 10.1107/s0907444901001214